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miR-338-5p Levels and Cigarette Smoking are Associated With Neuropathic Pain Severity in Individuals With Spinal Cord Injury: Preliminary Findings From a Genome-Wide microRNA Expression Profiling Screen

Published:October 27, 2021DOI:https://doi.org/10.1016/j.apmr.2021.09.005

      Abstract

      Objective

      To identify microRNA biomarkers and clinical factors associated with neuropathic pain after spinal cord injury.

      Design

      Cross-sectional, secondary analysis of baseline data collected from ongoing clinical studies. Using a genome-wide microRNA screening approach, we studied differential microRNA expression in serum from 43 adults with spinal cord injury enrolled in ongoing clinical studies. Least squares regression was used to identify associations between microRNA expression, clinical factors, and neuropathic pain severity.

      Setting

      Community-dwelling individuals with spinal cord injury.

      Participants

      Participants (N=43) were at least 18 years old with spinal cord injury, with 28 reporting neuropathic pain and 15 reporting no neuropathic pain.

      Interventions

      Not applicable.

      Main Outcome Measures

      Pain presence, type, and intensity were assessed with the International Spinal Cord Injury Pain Basic Data Set. Serum microRNA normalized deep sequencing counts were quantified from blood samples. Participant demographic factors, injury characteristics, medication use, and health habits were collected via questionnaire.

      Results

      miR-338-5p expression and history of cigarette smoking were associated with and explained 37% of the variance in neuropathic pain severity (R2=0.37, F2,18=5.31, P=.02) independent of other clinical factors. No association was identified between miR-338-5p levels and nociceptive pain severity.

      Conclusions

      Our findings suggest that miR-338-5p and cigarette smoking may both play a role in the development or maintenance of neuropathic pain after spinal cord injury. While additional work is needed to confirm these findings, validated target analysis suggests a neuroprotective role of miR-338-5p in modulating neuroinflammation and neuronal apoptosis and that its downregulation may result in maladaptive neuroplastic mechanisms contributing to neuropathic pain after spinal cord injury.

      Keywords

      List of abbreviations:

      AIS (American Spinal Injury Association Impairment Scale), BCL2L11 (Bcl-2-like protein 11), CTGF (connective tissue growth factor), CXCR4 (chemokine (C-X-C motif) receptor 4), IL (interleukin), ISCIPBDS (International Spinal Cord Injury Pain Basic Data Set), miRNA (microRNA), PHQ-9 (Patient Health Questionnaire-9), SCI (spinal cord injury)
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