Archives of Physical Medicine and Rehabilitation
Volume 90, Issue 1 , Pages 66-73 , January 2009

Functional Overloading of Dystrophic Mice Enhances Muscle-Derived Stem Cell Contribution to Muscle Contractile Capacity

Presented as an abstract to Engineering Tissues: Replace, Repair, Regenerate, March 8–11, 2007, Hilton Head, SC.

  • Fabrisia Ambrosio, PhD, MPT

      Affiliations

    • Departments of Physical Medicine and Rehabilitation, Pittsburgh, PA
    • Stem Cell Research Center, Children's Hospital of Pittsburgh, Pittsburgh, PA
    • Corresponding Author InformationReprint requests to Fabrisia Ambrosio, PhD, MPT, Dept of Physical Medicine & Rehabilitation, Ste 202, 3471 5th Ave, Pittsburgh, PA 15213
    • ,
  • Ricardo J. Ferrari, MS

      Affiliations

    • Departments of Physical Medicine and Rehabilitation, Pittsburgh, PA
    • Stem Cell Research Center, Children's Hospital of Pittsburgh, Pittsburgh, PA
    • ,
  • G. Kelley Fitzgerald, PT, PhD, OCS

      Affiliations

    • Physical Therapy, Pittsburgh, PA
    • ,
  • George Carvell, PhD, PT

      Affiliations

    • Physical Therapy, Pittsburgh, PA
    • ,
  • Michael L. Boninger, MD

      Affiliations

    • Departments of Physical Medicine and Rehabilitation, Pittsburgh, PA
    • ,
  • Johnny Huard, PhD

      Affiliations

    • Orthopedic Surgery, University of Pittsburgh, Pittsburgh, PA
    • Stem Cell Research Center, Children's Hospital of Pittsburgh, Pittsburgh, PA

  • Image Result

    An animal grouping schematic.

    An animal grouping schematic.

  • Image Result

    A contractile testing schematic representation.

    A contractile testing schematic representation.

  • Image Result

    Contractile testing: specific twitch (Pt/CSA) and tetanic (Po/CSA) forces among 4 groups. NOTE. Mean N/cm2 ± SD, n=5 per group. *Denotes statistically significant from OL/ PBS (P≤.05). †Denotes statis

    Contractile testing: specific twitch (Pt/CSA) and tetanic (Po/CSA) forces among 4 groups. NOTE. Mean N/cm2 ± SD, n=5 per group. *Denotes statistically significant from OL/ PBS (P≤.05). †Denotes statistically different from OL/ MDSC (P≤.05).

  • Image Result
    Immunofluorescence of dystrophin (red) and nuclei (blue) in the EDL of (A) MDSC-only and (B) OL/MDSC muscles. Green represents fluorescent microspheres used to localize injection site (20× magnificati

    Immunofluorescence of dystrophin (red) and nuclei (blue) in the EDL of (A) MDSC-only and (B) OL/MDSC muscles. Green represents fluorescent microspheres used to localize injection site (20× magnification).

  • Image Result
    Comparison of the efficacy of stem cell engraftment (as determined by the number of dystrophin-positive fibers/cross-sectional area) for MDSC-only and OL/MDSC muscles (n=5). NOTE. Mean ± SD. *Denotes

    Comparison of the efficacy of stem cell engraftment (as determined by the number of dystrophin-positive fibers/cross-sectional area) for MDSC-only and OL/MDSC muscles (n=5). NOTE. Mean ± SD. *Denotes statistically significant P≤.05.

 Supported in part by the Competitive Medical Research Fund of the University of Pittsburgh; the K12 for Physical and Occupational Therapists–A Comprehensive Opportunities in Rehabilitation Research Training program (grant no. 1K12HD055931-01); the Henry J. Mankin Endowed Chair for the Orthopedic Research at the University of Pittsburgh; the William F. and Jean W. Donaldson Chair at Children's Hospital of Pittsburgh, Pittsburgh, PA; and the NIH (grant no. 5R01 AR49684).

 An organization with which 1 or more of the authors is associated has received or will receive financial benefits from a commercial party having a direct financial interest in the results of the research supporting this article.

PII: S0003-9993(08)01540-2

doi: 10.1016/j.apmr.2008.06.035

Archives of Physical Medicine and Rehabilitation
Volume 90, Issue 1 , Pages 66-73 , January 2009

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